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Бюллетень сибирской медицины

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Двухмерные и трехмерные модели культур клеток опухолей in vitro: преимущества и недостатки

https://doi.org/10.20538/1682-0363-2018-3-188-196

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Аннотация

Синтез и внедрение новых химических соединений, обладающих потенциальной противоопухолевой активностью, требуют надежных предиктивных доклинических моделей для скрининга эффективности in vitro. Такие модели включают культуры клеток опухолей человека – двухмерные системы культур клеток 2D (two-dimensional cell culture systems) и трехмерные системы культур клеток 3D (three-dimensional cell culture systems). В этом обзоре обсуждаются особенности молекулярного фенотипа клеток, культивируемых в 2Dand 3D-системах, и их применение в исследованиях эффективности противоопухолевых препаратов с упором на ключевые примеры из научной литературы. В обзоре также проанализированы преимущества, недостатки и перспективы применения описываемых моделей культур опухолевых клеток.

Об авторах

Э. С. Галимова
Национальный медицинский исследовательский центр (НМИЦ) им. В.А. Алмазова
Россия

Галимова Эльвира Сафуановна - кандидат биологических наук, старший научный сотрудник, отдел биохимических исследований, Центр доклинических и трансляционных исследований, Институт ýкспериментальной медицины.

197341, Санкт-Петербург, ул. Аккуратова, 2



М. М. Галагудза
Национальный медицинский исследовательский центр (НМИЦ) им. В.А. Алмазова
Россия

Галагудза Михаил Михайлович - докторр медицинских наук, член-корр. РАН, директор Института ýкспериментальной медицины.

197341, Санкт-Петербург, ул. Аккуратова, 2



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Для цитирования:


Галимова Э.С., Галагудза М.М. Двухмерные и трехмерные модели культур клеток опухолей in vitro: преимущества и недостатки. Бюллетень сибирской медицины. 2018;17(3):188-196. https://doi.org/10.20538/1682-0363-2018-3-188-196

For citation:


Galimova E.S., Galagudza M.М. Two-dimensional and three-dimensional cell culture models in vitro: pros and cons. Bulletin of Siberian Medicine. 2018;17(3):188-196. (In Russ.) https://doi.org/10.20538/1682-0363-2018-3-188-196

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ISSN 1682-0363 (Print)
ISSN 1819-3684 (Online)